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Title

Molecular Mechanisms of Luteolin Induced Growth Inhibition and Apoptosis of Human Osteosarcoma Cells

Pages

 Start Page 531 | End Page 538

Abstract

Luteolin is a flavone in medicinal plants as well as some vegetables and spices. It is a natural Anti-oxidant with less pro-oxidant potential but apparently with a better safety profile. The purpose of this study was to investigate the molecular mechanism of Luteolin-mediated Apoptosis of MG-63 human Osteosarcoma cells. MTT assay kit was employed to evaluate the effects of Luteolin on MG-63 cells Proliferation. Then, we performed Annexin V-FITC/PI to analyze the apoptotic rate of the cells. Furthermore, the inhibitory effects of Luteolin on the expressions of BCL-2, BAX, Caspase-3 and Survivin were detected by Western blotting. As expected, Luteolin (0. 5, 2. 5, 12. 5 μ g/mL) inhibited the growth of MG-63 cells by inhibiting cell Proliferation and inducing cell Apoptosis. Western blotting demonstrated that Luteolin (0. 5, 2. 5, 12. 5 μ g/mL) inhibited the expressions of BCL-2, Caspase-3 and Survivin, and promoted the expression of BAX in MG-63 cells with a concentration dependent way. Luteolin can inhibit osteosarcoma cell Proliferation and induce Apoptosis effectively in a dose dependent manner through down-regulating the expression of BCL-2, Caspase-3 and Survivin proteins levels and up-regulating the expression of BAX protein level. These findings indicated that Luteolin may be used as a novel herbal medicine for the treatment of osteosarcoma.

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