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Title

STUDY ON THE MULTI-MARKER COMPONENTS QUANTITATIVE HPLC FINGERPRINT OF THE COMPOUND CHINESE MEDICINE WUWEI CHANGYANNING GRANULE

Pages

 Start Page 1191 | End Page 1201

Abstract

 The aim of this paper is to develop a rapid and highly sensitive quantitative HPLC FINGERPRINT method with multiple indicators by using the Compound Chinese Medicine WUWEI CHANGYANNING GRANULE and 5 herbs in the prescription. The quantitative fingerprint chromatogram with multiple indicators was investigated. i) 6 compositions included rutin, gallic acid, chlorogenic acid, atractylenolide i, pachymic acid and apigenin, which originated from 5 herbs respectively, were selected as quantitative compositions, and their contents were determined using HPLC from 11 batches granules and the corresponding 5 medicinal materials. ii) The precision, stability and repeatability of fingerprinting were investigated. In addition, COMMON PEAKS NUMBER, THE PERCENTAGE OF NON-COMMON PEAKS AND SIMILARITY were also studied. Among them, 21 common peaks in the granule could find the source of peaks from the 5 herbs, among of 10 peaks from Niuerfeng, 9 peaks from Laliao, 3 peaks from Baishu, 3 peaks from Fuling and 5 peaks from Guanghuoxiang. The results showed that the identification method of fingerprinting was reliable.

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