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Title

DIRECT ELECTROCHEMISTRY OF HEMOGLOBIN AND ITS BIOSENSING FOR HYDROGEN PEROXIDE ON TIO2–POLYSTYRENE NANOFILMS

Pages

 Start Page 1569 | End Page 1577

Keywords

TIO2 NANOPARTICLES 

Abstract

 Nanofilms of titanium dioxide (TiO2) nanoparticles that mediate the assembly of POLYSTYRENE (PS) nanoparticles for immobilizing HEMOGLOBIN (Hb) on carbon ionic liquid electrode have been developed. Fourier transform infrared spectroscopy shows that Hb retains its native structure in TiO2-PS nanofilms. Scanning electron microscopy reveals that the nanofilms possess uniform morphology and Hb is immobilized on the surface of the nanofilms. Electrochemical investigation of the BIOSENSOR indicates that the DIRECT ELECTROCHEMISTRY of HEMOGLOBIN is realized on the nanofilms, and there is a formal potential of -0.320 V in deaerated buffer solutions; the BIOSENSOR shows good electrocatalytic activity toward the reduction of hydrogen peroxide with a linear range from 0.5 to 640 mM, a detection limit of 0.2 mM (S/N = 3) and a sensitivity of 103 mA mM-1. Thus, the nanofilms will have potential application in the design of novel electrochemical BIOSENSORs.

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