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Title

Evaluation of miR-182/miR-100 Ratio for Diagnosis and Survival Prediction in Bladder Cancer

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 Start Page 645 | End Page 651

Abstract

 BACKGROUND: Abnormal expression of microRNAs (miRNAs) plays an important role in development of several cancer types, including bladder cancer (BCa). However, the relationship between the ratio of miR-181/miR-100 and the prognosis of BCa has not been studied yet. The aim of this study was to evaluate the expression of miR-182, miR-100 and their clinical significance in BCa. METHODS: Upregulation of miR-182 and down-regulation of miR-100 were validated in tissue specimens of 134 BCa cases compared with 148 normal bladder epithelia (NBE) specimens using TaqMan-based real-time reverse transcription quantitative PCR (RT-qPCR). The diagnostic and prognostic evaluation of miR-182, miR-100, and miR-182/miR-100 ratio was also performed. RESULTS: miR-182 was upregulated in BCa and miR-100 was down-regulated in BCa compared with NBE (P < 0. 001). The areas under receiver operating characteristic curves (AUCs-ROC) for miR-182 and miR-100 were 0. 913 and 0. 810, respectively. However, miR-182/miR-100 ratio increased the diagnostic performance, yielding an AUC of 0. 981 (97. 01% sensitivity and 90. 54% specificity). Moreover, miR-182/miR-100 ratio was associated with pT-stage, histological grade, BCa recurrence and carcinoma in situ (P < 0. 05 for all). Multivariate Cox regression analysis indicated that miR-182/miR-100 ratio was an independent prognostic factor for overall survival (Hazard ratio: 7. 142; 95% CI: 2. 106 – 9. 891; P < 0. 01). Furthermore, Kaplan-Meier curve analysis revealed that high-level of miR-182/miR-100 ratio was significantly correlated with shortened survival time for BCa patients (P < 0. 01). CONCLUSION: The miR-182/miR-100 ratio may serve as a novel promising Biomarker for diagnosis and survival prediction in BCa. Further studies are needed to elucidate the role of miR-182/miR-100 ratio as a non‑ invasive diagnostic tool for BCa.

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