video

sound

Persian Version

View:

70,235

Download:

24,690

Cites:

Information Journal Paper

Title

FUNCTIONAL AND DEVELOPMENTAL ANALYSIS OF CD4+CD25+ REGULATORY T CELLS UNDER THE INFLUENCE OF STREPTOCOCCAL M PROTEIN IN RHEUMATIC HEART DISEASE

Pages

  122-127

Keywords

CD4+CD25+ REGULATORY T CELLS 

Abstract

 The purpose of this study was to determine the role of streptococcal M protein in naturally-occurring CD4+CD25+ regulatory T cells (nTregs) function and development in RHEUMATIC HEART DISEASE in Iraqi patients. Streptococcus pyogenes was isolated for subsequent M protein extraction. Also, peripheral blood nTregs and CD4+ T cells were isolated by using Magnetic Cell Separation System. Tissue culture for isolated cells was performed in the presence and absence of M protein. Cell count was performed, and tumor necrosis factor alpha (TNF-a) and interleukin-4 (IL-4) were determined in culture supernatant using ELISA system. There was a significant positive correlation (P<0.01) between the number of proliferated nTregs and CD4+ T cells in the presence as well as the absence of streptococcal M protein. Moreover, there was a significant negative correlation between the mean number of nTregs and CD4+ T cells in mixed culture system in the absence of M protein (r=- 0.995). There was also a positive, but not significant (P>0.05), association (r=0.353) between the mean number of nTregs and CD4+ T cells in the presence of M protein. The M protein stimulated CD4+ T cells to produce IL-4 in very little amount (<4 pg/ml) in all samples. Compared to the production of IL4, TNF-a was produced in higher concentrations in the culture supernatants. The findings of the study indicate that streptococcal M protein has an important role in increasing the proliferation of CD4+CD25+regulatory T cellsand CD4+ T cells. However, CD4+CD25+ regulatory T cells have lower suppressive activity against CD4+ T cells in the presence of M protein.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    ABDUL AUHAIMEN, N., & AL KAABI, Z.I.. (2011). FUNCTIONAL AND DEVELOPMENTAL ANALYSIS OF CD4+CD25+ REGULATORY T CELLS UNDER THE INFLUENCE OF STREPTOCOCCAL M PROTEIN IN RHEUMATIC HEART DISEASE. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS), 36(2), 122-127. SID. https://sid.ir/paper/276385/en

    Vancouver: Copy

    ABDUL AUHAIMEN N., AL KAABI Z.I.. FUNCTIONAL AND DEVELOPMENTAL ANALYSIS OF CD4+CD25+ REGULATORY T CELLS UNDER THE INFLUENCE OF STREPTOCOCCAL M PROTEIN IN RHEUMATIC HEART DISEASE. IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS)[Internet]. 2011;36(2):122-127. Available from: https://sid.ir/paper/276385/en

    IEEE: Copy

    N. ABDUL AUHAIMEN, and Z.I. AL KAABI, “FUNCTIONAL AND DEVELOPMENTAL ANALYSIS OF CD4+CD25+ REGULATORY T CELLS UNDER THE INFLUENCE OF STREPTOCOCCAL M PROTEIN IN RHEUMATIC HEART DISEASE,” IRANIAN JOURNAL OF MEDICAL SCIENCES (IJMS), vol. 36, no. 2, pp. 122–127, 2011, [Online]. Available: https://sid.ir/paper/276385/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops