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Title

THE PREVALENCE OF ESCHERICHIA COLI STRAINS HARBORING IPAH VIRULENCE GENE ISOLATED FROM SURFACE WATER SOURCES OF TEHRAN PROVINCE

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 Start Page 134 | End Page 134

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PCR 

Abstract

 Background: E. COLI is considered as one of the most important causes of bacterial infections transmitted through WATER and foods. Several factors are involved in the pathogenesis of E. COLI including IPAH gene related to entroinvasive pathotype. The aim of this study was to investigate the prevalence of E. COLI strains carrying virulence gene IPAH isolated from different surface WATER sources in Alborz province.Methods: This study was carried out in 2013. The research included all E. COLI strains isolated from different surface WATER sources of Tehran province including input of 5 WATER treatment centers. E. COLI isolates were detected and identified by standard microbiological and biochemical tests. The strains were evaluated for presence of virulence gene IPAH by PCR using specific primers. The PCR amplicons were visualized after electrophoresis and staining with SYBR green.Results: One hundred and six E. COLI strains were isolated and included in the study. The PCR results showed 4 strains harbored IPAH gene. Approximately, we found an equal rate of ETEC detection among the strains isolated from different WATER source. Most of bacterial strains harboring IPAH gene were MDR.Conclusion: Our finding showed the prevalence of virulence gene IPAH is not so much high among E. COLI strains isolated from different surface WATER sources in Tehran province. Considering its plasmid borne nature, the risk of transmission of this gene between other bacterial species could pose a high threat for public health.

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