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Title

VIRULENCE CHARACTERISTICS OF E.COLI ISOLATED FROM CHILDREN WITH URINARY TRACT INFECTION

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 Start Page 107 | End Page 107

Abstract

 Background: Uropathogenic Escherichia coli (UPEC) is a causative agent in most urinary tract infections (UTIs), expressing a multitude of virulence factors. The aim of this study was to investigate some characteristics in UPEC isolates derived from urine specimens.Methods: A total of 50 E. coli isolates were collected from patients suffering from UTI during September 2009 to September 2010.Biochemical and standard microbiological techniques were used to identify E. coli and then screened for phylogenetic typing groups, pathogenicity islands, hlyD and intI1 genes by polymerase chain reaction (PCR).Results: We found a high number of PAI markers such as PAI ICFT073, PAI IICFT073, PAI I536, PAI IV536, PAI II J96, and PAI II536 significantly associated with UPEC. PCR phylogenetic typing groups revealing that the higher prevalence of uropathogenic strains were mainly found in subgroup B2 and D. High resistance to aztreonam, cotrimoxazole, cefpodoxime, and cefotaxime were found in UPEC isolates. It was shown that hlyD was present in 26% of UPEC, however, hemolysin was expressed as 42% (p<0.05). The IntI1 gene was expressed as 24%.Conclusion: Knowledge of the molecular details of uropathogenic E. coli is useful for development of successful strategies for treatment of urinary tract infection in human and complications associated with UTIs.

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