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Title

STUDY OF AMYLASE-PRODUCING BACTERIA BIODIVERSITY IN THE DRILLING FLUID

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 Start Page 53 | End Page 53

Abstract

 Background: BIODEGRADATION of STARCH used in DRILLING FLUID by many microorganisms, results in reduction of STARCH concentration in the fluid, which leads to fluid efficiency downfall. The aim of present study was to identifythe major STARCH degrading bacteria in the DRILLING FLUID and manner of controling the growth and activity of these organisms.Methods: Bacteria present in back up DRILLING FLUID, were isolated and their amylase activity was studied by STARCH hydrolysis test. The strain with the most ability of STARCH hydrolysis in all three temperatures (37, 45 and 55oC) was selected as the superior strain and was identified by 16Sr RNA. Also growth curve of superior strain was plotted.Results: 54 bacterial strains include 26 Gram-positive Bacillus, 22 Gram-negative Bacillus and 6 Gram-positive Cocci were isolated. 18 strains from 26 Gram-positive Bacillus were spore forming bacteria. Three strains had growth in all three temperatures (37, 45 and 55oC) and showed significant amylase activity. Bacteria with the most mean of inhibition zone were selected as superior strains. The results of the 16Sr RNA analysis showed 99.8% similarity of superior strain to Bacillus licheniformis. The bacterium was in logarithmic phase at 8-48 hour after inoculation.Conclusion: Spore forming Gram-positive Bacillus belonging to Bacillus Genus like Bacillus licheniformis isolated in this study was the most active bacteria of degrading in well special conditions. Identification of degradation factors with the aim of selecting a suitable biocide is important to prevent the BIODEGRADATION.

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