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Title

COMPARISON OF THE ANTIBACTERIAL EFFECTS OF THREE ENDODONTIC ROOT CANAL SEALERS (AH26, AH PLUS AND MTA FILLAPEX) ON ENTEROCOCCUS FAECALIS

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 Start Page 32 | End Page 32

Abstract

 Background: Successful endodontic treatment requires effective removal of the bacteria from the mechanically formed canal. Sealers used to fill the canal should also have antibacterial properties in order to prevent bacterial growth. In this study the ANTIBACTERIAL EFFECT of three ROOT CANAL SEALERS ; AH26, AH Plus and MTA Fillapex is compared using E. faecalis as the reference organism, which is one of the most resistant bacteria causing root canal treatment failure.Methods: Five plates of Muller-Hinton agar were simultaneously inoculated with a standard solution of E.faecalis (ATCC 29212). Each plate was divided into 3 equal parts with a 6mm well in each section. Each well was used for one type of sealer. Freshly prepared sealers were then poured into their wells. The diameter of inhibition zone around each sealer was measured 24, 48 and 72 h after incubation at 37 O C. The entire experiment was repeated three times and the data was analyzed using ANOVA and Tukey with SPSS 19.Results: Mean and SD of the diameter of the inhibition zones for AH26, AH plus and MTA Fillapex were 16.2, 1.014, 10.33, 0.617 and 6.67, 0.9 mm respectively. The observed difference was significant (P=0.013). There were no significant differences in three runs for each of the sealers.Conclusion: AH26 had the highest ANTIBACTERIAL EFFECT AH plus was also effective but MTA Fillapex had no ANTIBACTERIAL EFFECT at all.

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