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Title

ISOLATION AND MOLECULAR IDENTIFICATION OF L-ASPARAGINASE PRODUCING BACTERIA FROM PERSIAN GULF

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 Start Page 133 | End Page 133

Abstract

 Background: L-ASPARAGINASE is an anti-neoplastic agent used in the chemotherapy of lymphoblastic leukemia. Nowadays, this enzyme is derived from bacterial sources. The objective of this study was isolation and molecular identification of Lasparaginase producing bacteria from marine environment in PERSIAN GULF.Methods: 53 MARINE BACTERIA were isolated from sediment and seawater samples obtained from PERSIAN GULF in Hormozgan, Iran. The isolated strains were screened for Lasparaginase activity. Production of L-ASPARAGINASE was carried out in medium M9. Based on the enzyme activity among 20 strains producing L-ASPARAGINASE, 4 isolates with high Lasparaginase activity were selected and identified base on nucleotide sequence of 16S RRNA gene.Results: 4 bacteria isolated were identified as Pseudomonas strains and four strains of Bacillus sp. and Pseudomonas sp. (PG01) showed the highest productivity of 1.6 IU, and Bacillus sp. (PG04) showed least productivity of 0.67 IU.Conclusion: This study revealed that bacteria isolated from PERSIAN GULF may be potential source of enzyme Lasparaginase and Pseudomonas sp. (PG01) with high productivity L-ASPARAGINASE can used for chemotherapy of acute lymphoblastic leukemia, biosynthesis of the aspartic family of amino acids and production decrease acrylamide in food industrial.

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