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Title

ACTINOMYCETES WITH AMYLOLYTIC ACTIVITY ISOLATED FROM SEDIMENTS OF THE CASPIAN SEA

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 Start Page 132 | End Page 132

Abstract

 Background: The aims of this study were isolation and characterization of actinomycetes with amylolyticactivity from sediment of the CASPIAN SEA.Methods: For isolation of actinomycetes, sediments were collected in different depth of water. Plates were incubated for 21 days at 28oC after inoculation of samples into Starch Casein Agar (SCA) as a selective medium. Colonies with a tough or powdery texture were isolated. Primary screening was performed by the starch plate assay method and amylolytic activity of isolates was confirmed by formation of clear zones of hydrolysis around the colonies. It was also determined by DNS (3, 5-dinitrosalicylic acid) assay method as secondary screening.Results: Of the sediments, 14actinomycetes were isolated and identified.All of them were determinedas active actinomycetes in primary screening. Among thesebacteria, five isolatesshowed good ability to produce amylasein secondary screening.Conclusion: The result of this study demonstrated that the sediments of the CASPIAN SEA have the potential to be a diversity of actinomycetes with amylolytic activity.

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