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Title

ISOLATION OF MODERATELY HALOPHILIC BACTERIA PRODUCING PULLULANASE ENZYME FROM DEGH BIARJEMAND DESERT OF SHAHROD, IRAN

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 Start Page 125 | End Page 125

Abstract

 Background: The main objective of this study was to isolate and identify moderately HALOPHILIC BACTERIA that produce saline- tolerant PULLULANASE ENZYME in hypersaline condition.Methods: The bacteria were isolated by saline culture from saline soils in Degh Biarjemand desert of Shahrod. Then moderately HALOPHILIC BACTERIA were isolated. On the next step, the isolated HALOPHILIC BACTERIA were analyzed for production of pullulanse enzyme. After that, they were identified by molecular and biochemical methods.Results: A total of 11 strains were isolated from Biarjemand desert of Shahrod. 9 out of the 11 HALOPHILIC BACTERIA were moderately halophilic and the two others were halotolerant, but no extreme halphilic bacteria were among the isolated strains. The HALOPHILIC BACTERIA producing hydrolytic enzymes were analyzed. 2 strains of the bacteria produced pullulanase which one of them was moderately halophilic bacterium. The halophilic bacterium producing pullulanse enzyme was identified concerning the 16S rRNA sequencing technique as well as it’s molecular and biochemical properties showed that the isolate belongs to the genus Bacillus.Conclusion: In this study, for the first time, the HALOPHILIC BACTERIA from Degh Biarjemand desert of Shahrod were isolated and analyzed for production of PULLULANASE ENZYME and special HALOPHILIC BACTERIA were isolated. The isolated halophilic bacterium produced saline-tolerant PULLULANASE ENZYME, was tolerant and active in hypersaline condition.

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