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Title

ANTIMICROBIAL RESISTANCE AND CLASS 1 INTEGRON IN ESCHERICHIA COLI STRAINS ISOLATED FROM DIFFERENT ANIMAL SOURCES

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 Start Page 90 | End Page 90

Abstract

 Background: The aim of the current study was to evaluate the prevalence of CLASS 1 INTEGRONs associated ANTIBIOTIC RESISTANCE among E. COLI strains isolated from different animal sources.Methods: E. COLI strains isolated from different animal sources including human, hen, cow and sheep were included in this study. Bacterial strains were isolated and identified by standard microbiological and biochemical tests. The antimicrobial susceptibility testing was performed according to Kirby Baur assay using 11 antibiotic discs including Amikacin, Co-trimoxazole, Tobramycin, Stereptomycin, Piperacillin, Ampicillin, Cefazolin, Nalidixic acid, Gentamicin, Kanamycin, and Neomycin. Total genomic and plasmid DNAs were extracted using AccuPrepÒ Genomic DNA Extraction Kit. To detect the CLASS 1 INTEGRON, a newly designed int1 specific primer was used for amplification of integrase gene by PCR. The PCR amplicons were visualized after electrophoresis and staining with SYBR green.Results: Eighty E. COLI strains were isolated and included in this study. Antibiotic susceptibility testing showed that 59% of the isolates were MDR while 31% harbored the int1gene. Following statistical analysis, chi–square test and p value determination, we found a significant relation between the presence of CLASS 1 INTEGRON and resistance to Cefazolin, Sulfamethoxazole, Nalidixic acid, Gentamicin and Kanamycin resistance.Conclusion: Result from current research indicates high prevalence of antibiotic resistant E. COLI strains isolated from different animal sources. Our results also confirm the significant role of CLASS 1 INTEGRON to create and facilitate the spread of ANTIBIOTIC RESISTANCE among E. COLI strains.

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