video

sound

Persian Version

View:

138

Download:

0

Cites:

Information Journal Paper

Title

FFECT OF RAMADAN FASTING ON PARAOXONASE/ ARYLESTERASE ACTIVITIES, LDL OXIDIZABILITY, AND VITAMIN E CONTENT OF SERUM LDL IN NORMOCHOLESTEROLEMIC AND HYPERCHOLESTEROLEMIC SUBJECTS

Pages

 Start Page 16 | End Page 16

Keywords

Not Registered.

Abstract

 introduction: Recent studies have demonstrated that oxidized LOL plays an important role in the initiation and progression of atherosclerosis. Paraoxonase (PON) is an HOL-associated enzyme, which is believed to protect LOL against oxidative modification. Some studies suggest that vitamin E can protect LDL from oxidative damage in vitro. The purpose of this study is to determine the effects of Ramadan fasting on paraoxonase/ arylesterase activities, LOL oxidizability. and vitamin E content of serum LOL among normocholesterolemic and hypercholesterolemic subjects.Material & Methods: Sixteen normocholesterolemic (TC<200), 11 borderline (200<TC<240), and 14 hypercholesterolemic (TC³240 mg/ dl) volunteer subjects aged 25-50 years were recruited Blood samples were obtained on the 1st, 14th. and 29th days of the Ramadan. Measuring the initial rate of paraoxon hydrolysis to yield 4-nitrophenol-assessed Paraoxonase activity and arylesterase activity was determined by using phenyl acetate as the substrate. The LOL oxidation susceptibility was followed spectrophotometrically after addition of copper and was expressed as LOL lag time and propagation rate. The plasma antioxidant activity capacity was measured according to the ferric reducing ability of plasma (FRAP method).Results: Paraoxonase activity decreased during Ramadan fasting in all norrnocholesterolernic (55±29 on the 1st vs 51±25 U/ml on the 29th dav of Ramadan), borderline group (69±42 on 1st vs 62±37 on 29th), and hypercholesterolemic patients (58±30 on 1st VS 54±27 U/ml] on the 29th day) (p<0.05). Mean lag time of LDL oxidation also decreased during fasting period in normocholesterolemic (57±11 on 1st vs 51±11 on 14th and 48±8 minutes on the 29th), borderline group (58±11 on 1st vs 56±13 on 14th and 49±11 minutes on 29th), and hypercholesterolemic group (58±12 on 1st vs 53±9 on 14th and 47±5 minutes on the 29th day). Vitamin E content of LOL decreased only in normocholesterolemic group (3.9±1.4 on 1st vs 3.1± 1.0 on 14th and 2.9±0.6 mg/ml on the 29th day; p<0.05). Plasma antioxidant activity was reduced significantly in all three groups. Propagation rate showed no significant changes during the fasting period in any group Arylesterase activity increased in subjects with borderline cholesterol (168±34 on 1 st vs 215±66 U/ml on the 29th day) but remained unchanged in other groups.Conclusion: Paraoxonase activity and vitamin E content of LOL falls and LOL oxidation susceptibility rises during Ramadan fasting These changes are more remarkable in norrnocholestcrolemic patients.

Cites

  • No record.
  • References

  • No record.
  • Related Journal Papers

    Related Seminar Papers

  • No record.
  • Related Plans

  • No record.
  • Recommended Workshops






    File Not Exists.