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Information Journal Paper

Title

AN INVESTIGATION OF THE EFFECT OF COPPER NANOPARTICLES ON E.COLI GENOME BY RAPD MOLECULAR MARKERS

Pages

 Start Page 475 | End Page 487

Abstract

 Regarding the bacterial resistence of common antibiotecs and anti-microbial agents, a great number of studies have been conducted to discover the recent types of antimicrobial agents. With the recent developments in nanotechnology, copper has been extensively used as nanoparticles against all positive-gram and to negative-gram particles due to its low price and high anti-microbial activity. In the present study, copper oxide nanoparticles of (<20 nm) have been used to study its effect on the genome of ESCHERICHIA COLI strain O157: H7 as a model for Gram-negative bacteria. To this end, the bacteria were first treated with 30 and 60 mg/mL copper oxide nanoparticles at the intervals of 2, 4, and 24 hours. and then their DNA were extracted. In order to investigate the effects of copper oxide nanoparticles on the genome, the chain reaction techniques of (RAPD-PCR) was employed. Using the software NTSYS-PC, the results obtaind from electrophoresis of PCR products on agaros gel were analyzed. The results of the studiy revealed that copper oxide nanoparticles not only affects the growth of bacteris but also affect the sequencing of genomic DNA and leads to the changes of them in different points.

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