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Information Journal Paper

Title

PRODUCTION OF PHENOLIC COMPOUNDS IN HAIRY ROOTS CULTURE OF RADISH (RAPHANUS SATIVUS L.)

Pages

 Start Page 327 | End Page 335

Abstract

AGROBACTERIUM RHIZOGENEScauses HAIRY ROOT disease in plants. The HAIRY ROOTs produced byA. rhizogenes infection are characterized by high growth rate and genetic stability. HAIRY ROOT induction cause changes in plant Secondary metabolites production. Phenolic compounds (Flavonoids, Tannins and Anthocyanins) are important antioxidants in radish. In this work, for induction of HAIRY ROOTs, leaf explants transformed with twoA. rhizogenesstrains (A4 and 15834 (GUS)). Generated HAIRY ROOTs confirmed with PCR. Also, Phenolic compounds content in HAIRY ROOTs was measured. Amplification of 780bp fragment forrol B and 320bp for GUS in TRANSGENIC roots confirmed the production of HAIRY ROOTs. Difference of Phenolic compounds between TRANSGENIC and non-transgenic roots was significant. Our results indicate that the insertion of A. rhizogenes T-DNA in to the plant genome stimulate plant defense responses and increase the production of Phenolic compounds.

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