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Title

OPTIMIZATION OF YARROWIA LIPOLYTICA LIPASE PRODUCTION BY TAGUCHI EXPERIMENT DESIGN METHOD

Pages

  336-343

Abstract

 Introduction: LIPASE (EC 3.1.1.3) is one of the most important classes of industrial enzymes. The non-conventional yeastYarrowia lipolytica is non-pathogenic to humans and is known as safe for some industrial processes. This yeast can produce different types of LIPASE. Extracellular LIPASE production depends on medium composition and environmental conditions. Materials and Methods: Olive oil as carbon source, yeast extract and tryptone as nitrogen sources and pH in different levels were optimized for LIPASE production by mutant strainYarrowia lipolytica FDY1390 with Taguchi EXPERIMENT DESIGN method. Qualitek-4 software was used to Taguchi EXPERIMENT DESIGN method. Results: The highest level of LIPASE production (340 U/mL) was obtained in medium containing 20 g/L Olive oil, 15 g/L yeast extract and tryptone, pH 4 after 72 h of fermentation process. Conclusion: OPTIMIZATION of LIPASE production reduces the costand various industries use this enzyme.

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