THE EFFECTS OF SUB LETHAL CONCENTRATIONS OF ENDOSOLFAN ON MRNAHSP70 EXPRESSION LEVEL AND GILL HISTOPATHOLOGY OF PERSIAN STURGEON (ACIPENSER PERSICUS BORODIN, 1897)

Writers

SAFARI R. | SHABANI A. | RAMEZANPOUR S. | KOLANGI H.

Keywords

PERSIAN STURGEONQ2

ENDOSULFANQ2

HEAT SHOCK PROTEINQ2

GENE EXPRESSIONQ1

HISTOPATHOLOGYQ2

Abstract

The increasing level of pollutants such as pesticides has destructive impact on ecosystems and aquatic organisms. Juveniles of Acipenser persicus were exposed to sub lethal concentrations of ENDOSULFAN (10, 20 and 40 mg/l) during 14 days. For GENE EXPRESSION study sampling were done from the both liver and gill on day 1, 2, 7 and 14. Result showed that in gill and liver, the relative HSP70-mRNA expression level significantly increased (P<0.05) in all studied days compare to control. Relative HSP70- mRNA showed a clear time- dependent response in both tissues after exposure to ENDOSULFAN. A significant increase (P<0.05) were observed on the second day, then decreased on day 7 and increased on day 14. The same trends were observed in all studied concentrations (10, 20 and 40 mg/l), in both examined tissues. In this study although up expression of HSP70- mRNA was common to both the gill and the liver, it significantly (P<0.05) expressed in the liver more than the gill. The amounts of mRNA expression were increased in the highest concentration in all studied days in this investigation. Histopatological study of gills showed that the intensity of lesions were increased gradually in all concentrations during the time exposure up to day 7 and then negligible changes in the index of lesions were observed on day 14 compare to day 7. The intensity of changes follows a concentration- dependent manner and 40 mg/l-treated fish samples showed the sign of lesions more severely than 20>10 mg/l that verify the genetic results.

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Journal Paper

THE EFFECTS OF SUB LETHAL CONCENTRATIONS OF ENDOSOLFAN ON MRNAHSP70 EXPRESSION LEVEL AND GILL HISTOPATHOLOGY OF PERSIAN STURGEON (ACIPENSER PERSICUS BORODIN, 1897)