Journal Paper

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Journal: گیاه پزشکی
Year:1397 | Volume:7 | Issue:2
Start Page:207 | End Page:217

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Title

GENETIC DIVERSITY AND PATHOGENIC VARIABILITY AMONG CERCOSPORA BETICOLA SACC. ISOLATES CAUSING LEAF SPOT OF SUGAR BEET IN IRAN

Pages

 Start Page 207 | End Page 217

Abstract

 Cercospora leaf spot caused byCERCOSPORA BETICOLA has a great negative impact on yield and quality of sugar beet. In the present study, pathogenic and genotypic variation of 24C. beticola isolates collected from different regions of Iran were studied using RFLP of the Internal Transcribed Spacer (ITS-RFLP), and Random Amplified Polymorphic DNA (RAPDPCR).Pathogenic variability and genotype × isolate interaction were evaluated in greenhouse experiments on five sugar beet cultivars (FD0018, HM1836, Puma, Eudora and Monatuna). All of the 24 isolates tested were found to be pathogenic on the cultivars with significant variation in disease severity. Results of RAPD analysis showed wide DNA polymorphism among the IranianC. beticola isolates. Restriction pattern of the internal transcribed spacer of rDNA (ITS1-5.8-ITS4) was studied using three restriction endonucleases: EcoR1, Taq1, and Busr1. The length of undigested DNA fragment of all isolates was estimated to be 500bp without rDNA polymorphism after digestion withEcoR 1 (280, 270 bp), Taq 1 (330 bp) and Busr1 (240, 220, 90 bp). RAPD and ITS-RFLP markers showed the highest level of genetic diversity which confirms the variation inC. beticola detection.

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