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Title

OPTIMIZATIONS OF α-AMYLASE PRODUCTION BY RESPONSE SURFACE METHODOLOGY IN IMMOBILIZATION BACILLUS AMYLOLIQUEFACIENS ATCC 23350

Pages

 Start Page 77 | End Page 86

Abstract

 Introduction: Production of an endogenous α-amylase from BACILLUS AMYLOLIQUEFACIENS ATCC 23350 was studied and enhanced.Materials and methods: Protein and carbon sources were analyzed for free and immobilized bacterial cells and number of beads was considered for immobilized cells via one factor at a time methodforα-amylase production by BACILLUS AMYLOLIQUEFACIENS. Subsequently, optimization condition was employed solely for immobilized bacterial cells by RESPONSE SURFACE METHODOLOGY (RSM).Results: Peptone and rice starch showed to improve the α-amylase production in immobilized Bacillus cells. RSM generated a mathematical model explaining the optimum concentration of the efficient nutrients (139.35 g/l of rice starch and 80.00 g/l of peptone) leading to an optimum amylase production (205 U/ml).Discussion and conclusion: The statistical advance displayed significant outcomes to optimize the process parameters for maximal α-amylase production using BACILLUS AMYLOLIQUEFACIENS and gave permission to rapid screening of variables. RSM led to find out an immense improvement in enzyme activity (more than 90%: from 25 to 225 U/ml) for the first time.

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