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Title

ISOLATION AND IDENTIFICATION OF PHYTASE-PRODUCING STRAINS FROM SOIL SAMPLES AND OPTIMIZATION OF PRODUCTION PARAMETERS

Pages

 Start Page 109 | End Page 118

Abstract

 Introduction: PHYTASE can be used as a feed additive to catalyze the hydrolytic degradation of PHYTATE as the major storage form of natural phosphorus. PHYTASE is produced by a wide range of bacteria, fungi and yeasts. Isolation and identification of PHYTASE-producing strains from soil, is of great interest for commercial application in different industries. The aim of the current study was the isolation and identification of PHYTASE-producing strains from soil samples and optimizing the enzyme production.Materials and methods: For isolation and identification of PHYTASE-producing strains, soil samples were collected from farms near Qazvin. Diluted samples were spread onto PSM solid media and production of the clear zones about the colonies gave a visual indication of PHYTASE production. The selection of the best PHYTASE-producing strain was performed by measuring the enzyme activity in the liquid medium. The selected strain was identified by slide-culture technique and the effect of carbon source (phytate and wheat bran), pH and time of incubation were also investigated for optimal enzyme production.Results: In this study, a PENICILLIUM SP. was isolated from a soil sample near Qazvin and was selected as the best PHYTASE-producing strain. The maximum PHYTASE activity (171 U/ml) was obtained in the medium containing % 2 (w/v) PHYTATE, at pH 5, after 72 h of incubation. By using wheat bran as the source of carbon and PHYTATE, the maximum PHYTASE activity, which was 61.7 U/mL, was produced at pH 7 and after the same time of incubation.Discussion and conclusion: PENICILLIUM SP. isolated from a soil sample near Qazvin, was able to produce highly active PHYTASE in optimized environmental conditions, which could be a suitable candidate for commercial production of PHYTASE to be used as complement in poultry feeding industries.

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