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Title

ISOLATION AND IDENTIFICATION OF PHYTASE-PRODUCING STRAINS FROM SOIL SAMPLES AND OPTIMIZATION OF PRODUCTION PARAMETERS

Pages

  109-118

Abstract

 Introduction: PHYTASE can be used as a feed additive to catalyze the hydrolytic degradation of PHYTATE as the major storage form of natural phosphorus. PHYTASE is produced by a wide range of bacteria, fungi and yeasts. Isolation and identification of PHYTASE-producing strains from soil, is of great interest for commercial application in different industries. The aim of the current study was the isolation and identification of PHYTASE-producing strains from soil samples and optimizing the enzyme production.Materials and methods: For isolation and identification of PHYTASE-producing strains, soil samples were collected from farms near Qazvin. Diluted samples were spread onto PSM solid media and production of the clear zones about the colonies gave a visual indication of PHYTASE production. The selection of the best PHYTASE-producing strain was performed by measuring the enzyme activity in the liquid medium. The selected strain was identified by slide-culture technique and the effect of carbon source (phytate and wheat bran), pH and time of incubation were also investigated for optimal enzyme production.Results: In this study, a PENICILLIUM SP. was isolated from a soil sample near Qazvin and was selected as the best PHYTASE-producing strain. The maximum PHYTASE activity (171 U/ml) was obtained in the medium containing % 2 (w/v) PHYTATE, at pH 5, after 72 h of incubation. By using wheat bran as the source of carbon and PHYTATE, the maximum PHYTASE activity, which was 61.7 U/mL, was produced at pH 7 and after the same time of incubation.Discussion and conclusion: PENICILLIUM SP. isolated from a soil sample near Qazvin, was able to produce highly active PHYTASE in optimized environmental conditions, which could be a suitable candidate for commercial production of PHYTASE to be used as complement in poultry feeding industries.

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    APA: Copy

    MOHAMMADI, MASOUD, AZIN, MEHRDAD, & SEDIGHI, MAHSA. (2017). ISOLATION AND IDENTIFICATION OF PHYTASE-PRODUCING STRAINS FROM SOIL SAMPLES AND OPTIMIZATION OF PRODUCTION PARAMETERS. BIOLOGICAL JOURNAL OF MICROORGANISM, 6(23), 109-118. SID. https://sid.ir/paper/237451/en

    Vancouver: Copy

    MOHAMMADI MASOUD, AZIN MEHRDAD, SEDIGHI MAHSA. ISOLATION AND IDENTIFICATION OF PHYTASE-PRODUCING STRAINS FROM SOIL SAMPLES AND OPTIMIZATION OF PRODUCTION PARAMETERS. BIOLOGICAL JOURNAL OF MICROORGANISM[Internet]. 2017;6(23):109-118. Available from: https://sid.ir/paper/237451/en

    IEEE: Copy

    MASOUD MOHAMMADI, MEHRDAD AZIN, and MAHSA SEDIGHI, “ISOLATION AND IDENTIFICATION OF PHYTASE-PRODUCING STRAINS FROM SOIL SAMPLES AND OPTIMIZATION OF PRODUCTION PARAMETERS,” BIOLOGICAL JOURNAL OF MICROORGANISM, vol. 6, no. 23, pp. 109–118, 2017, [Online]. Available: https://sid.ir/paper/237451/en

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