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Title

OPTIMIZATION OF RECOMBINANT LACCASE PRODUCTION BY YARROWIA LIPOLYTICA IN A MEDIUM CONTAINING GLUCOSE AS CARBON SOURCE WITH TAGUCHI METHOD

Pages

 Start Page 49 | End Page 56

Abstract

 Introduction: LACCASEs (EC 1.10.3.2; benzenediol: oxygen oxidoreductase) are copper-containing oxidases that use molecular oxygen to oxidize various aromatic and non-aromatic compounds. LACCASE is applied in delignification of lignocellulosic compounds for production of bioethanol, bioremediation of industrial wastewaters especially textile, food industries, and making biosensors.Materials and methods: The TAGUCHI EXPERIMENTAL DESIGN METHOD was used for OPTIMIZATION of LACCASE production in recombinant strain YARROWIA LIPOLYTICA YL4. A L-16 Taguchi orthogonal array was used to optimize the carbon and nitrogen sources along with vitamin in four levels.Results: The results showed that glucose, ammonium chloride, yeast extract and thiamine have significant effects on the production of LACCASE, respectively. The LACCASE activity reached to 1.52 U/mL after OPTIMIZATION of medium which is 7.6-fold higher than un-optimized medium.Discussion and conclusion: According to the analysis of results, the TAGUCHI EXPERIMENTAL DESIGN METHOD is a successful approach to increase LACCASE and recombinant proteins production in Y. lipolytica.

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