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Title

STUDY ON EXPRESSION PROFILE AND REGULATION PATTERN OF SUGAR TRANSPORTER GENE (SUT1) DURING COLD ACCLIMATION IN TWO WINTER WHEATS (TRITICUM AESTIVUM L.)

Pages

 Start Page 478 | End Page 487

Abstract

 Cold acclimation leads to biochemical, physiological and metabolic changes in plants which regulated in gene expression level by non-freezing temperature (0-4oC). These changes improve plant capacity to survive under freezing temperature. CARBOHYDRATEs accumulation is one of the most important changes which regulates by alternation in expression of sugar transporter genes. In this paper, gene expression profile of one sugar transporter gene (SuT1) was studied in freezing tolerant and susceptible variety under COLD ACCLIMATION (2oC) by Quantitative real-time PCR. Results showed differential expression pattern of this gene. SuT1 gene showed 3 times up-regulation and 3 times down-regulation after 1 day COLD ACCLIMATION in freezing tolerant and susceptible variety, respectively. Evaluation of PROMOTER sequence of this gene confirms the existence of low temperature responsive elements (LTRE), sugar responsive elements (SURE), dehydration responsive elements (DRE) and many other regulatory elements. Therefore, SuT1 gene can be introduced as a COR candidate gene to induce freezing tolerance in wheat. It is probable, the up-regulation of SuT1 gene leads to accumulate CARBOHYDRATE and decrease following freezing damages in hard winter condition.

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