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Title

RELATIONSHIP BETWEEN ALKANES HYDROXYLASE GENE AND ANALYSIS OF ALIPHATIC COMPOUNDS

Pages

 Start Page 35 | End Page 41

Abstract

 Aim and Background: Today hydrocarbon contamination is a major environmental problem. Petroleum hydrocarbons are mix compounds divided into four groups: saturates, aromatics, resins and asphalten. Among various phase of crude-oil, alkane with mediumchain(C10-C20) are favorable substrate that are rapidlydegraded, although short chain alkanesare verytoxic whilelong chain(C20-C40)oneshave low solubility in water that reduce bioavailability andmakeresistant to biodegradation. In this research, for the isolation of aliphatic degrading bacteria samplingwas performedin petroleum reservoir waste water in Tehran, Kerman and Semnan and also soil contaminated tohydrocarbon. Material and Methods:Alkane degrading bacteria were isolated by enrichment in Bushnell-Hass medium with hexadecane as sole source of carbon and energy. ALKANE HYDROXYLASE GENE wasidentified all strain by PCR with specific primers.Results: In this study,15strainswere isolated from bacterial decomposition of ALIPHATIC COMPOUNDS, and only one of them, i.e. P2, did not grow on the ALIPHATIC COMPOUNDS(Hexadecane as the solecarbon source). Thismeansthere is nodegradation of hydro carbons by this bacteriawill take place by this bacteria; but other strains we reable to grow on hexadecane and ALKANE HYDROXYLASE GENE was confirmed by PCR.Conclusions: This experiment shows that bacteria have the ability to degrade alkanes which musthad alkanes hydroxylase gene, so, lack of this gene causes indissoluble of the ALIPHATIC COMPOUNDS.

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