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Title

DETECTION OF MYCOPLASMA AGALACTIAE BY CULTURE AND POLYMERASE CHAIN REACTION (PCR) METHODS FROM AFFECTED SHEEP TO CONTAGIOUS AGALACTIAE IN BAFT COUNTY

Pages

 Start Page 423 | End Page 430

Abstract

MYCOPLASMA AGALACTIAE is the etiological agent of contagious agalactia that is a serious disease affecting SHEEP and goat. It occurs mainly in Mediterranean country and western Asia including Iran. It is characterized by mastitis and decline and subsequent failure of milk production, arthritis, keratoconjuctivitis and in appetence. The aim of the study was to detection of MYCOPLASMA AGALACTIAE by culture and PCR method from affected SHEEP in Baft.This survey was on all SHEEP and consume 1 year in 4 seasons in Baft. Dubious herds were under purposive sampling and samples were including milk secretion, conjunctival swab and synovial fluid. These samples enriched in PPLO broth media and for Mycoplasma species detection cultured in PPLO agar media and were characterized by amplification of 16S ribosomal RNA genes showed specific amplicon at 163 bp in PCR method. Positive specimens examined for M. agalactiae detection by amplification of lipoprotein gene that showed specific amplicon at 375 bp. From 17 samples, 8 Mycoplasma species identify that at one sample M. agalactiae species detected and M. agalactiae confirmed in 12.5 percent of Mycoplasma samples. This is the first report of M. agalactiae detection from affected SHEEP by two methods: culture and PCR method in Iranian SHEEP.This study shown 87.5 percent of contagious agalactia agents in Baft County are related to other species. However, in different sources main agent of 90 percent of this infection was M. agalactiae species.

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