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Title

EXPRESSION ANALYSIS OF TRYPSIN GENE IN PANCREATIC TISSUE OF BROILER CHICKEN FED DIETS CONTAINING OAK ACORN

Pages

 Start Page 363 | End Page 370

Abstract

 The aim of this research was to investigate the expression of TRYPSIN gene in the pancreas tissue of BROILER chicken fed tannin containing OAK diets. In this experiment, three diets (control, 15% and 20% OAK) were fed to BROILERs from 1 to 42 days of age. On days 21 and 42, total RNA extracted from pancreatic tissue of 36 BROILER chicken (18 for each time, 6 for each treatment). To study of GENE EXPRESSION, the TRYPSIN expression was compared with b-actin gene as a reference gene. Data of GENE EXPRESSION analzed, using REST, V2.0.13 2009 software. No significant association was found between pancreases relative weight and different treatments on day 21, but the relative weight of pancreases on day 42 was significantly for increased in diet with 20 percent of OAK acorn in comparison to other treatments. The mRNA expression level of TRYPSIN gene was significantly higher in 20 percent of OAK acorn treatment on day 21, but their differences were not significant in compared to control diet on day 42. In addition, significant correlation was found between GENE EXPRESSION of TRYPSIN and relative weight of pancreases on day 42.

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