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Title

STUDY ON LEAD BIOSORPTION BY LEAD RESISTANT BACTERIA ISOLATED FROM AQQALA INDUSTRIAL PARK WASTEWATER

Pages

 Start Page 257 | End Page 267

Abstract

 Background & Objectives: LEAD is a heavy metal and persistent pollutant in the environment that enters through industrial wastewaters. The aim of this study was to isolate LEAD RESISTANT BACTERIA from the industrial wastewaters, and to study the LEAD absorption by the isolated bacteria.Materials & Methods: This descriptive study was performed on three wastewater samples. Nutrient agar medium containing different concentrations of LEAD acetate were used to isolate the LEAD RESISTANT BACTERIA. Overall, 28 colonies were selected and their characteristics were determined. Of these, six colonies with the highest LEAD resistance were selected. The rate of LEAD absorption of these strains was measured using atomic absorption spectrophotometry. Four strains with higher LEAD absorption was identified using molecular method.Results: 78.5% of the isolated RESISTANT BACTERIA were Gram positive and 21.5% of the bacteria were Gram negative bacteria. Biochemical tests showed that 46.5% of the isolates were belonged to Bacillus sp. Of these, T5 had the highest LEAD absorption. Also, sequencing results revealed that the selected isolates are belong to Escherichia coli strain 789, Enterobacter cloacae strain GGT036, Bacillus tequilensis strain KM30 and Kurthia sp. VITA1 (T5).Conclusion: The result of this study showed that the bacteria isolated from LEAD-containing wastewaters are highly potent for removal of this metal from polluted environments, and therefore can be appropriate candidates for BIOREMEDIATION and biological treatment.

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