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Title

ASSESSMENT OF CHEMICAL COMPOSITIONS AND ANTIBACTERIAL ACTIVITY OF THE EXTRACT AND ESSENTIAL OIL OF ARTEMISIA AUCHERI COLLECTED FROM IRAN

Pages

 Start Page 346 | End Page 354

Abstract

 Background & Objectives: The antimicrobial activity of plant oils and EXTRACTs has been recognized for many years. There are many difficulties and deficiencies to control plant photogenic bacteria. Historically, natural plant products have been considered as great sources with therapeutic properties. This study was aimed to evaluate the chemical compositions and antibacterial activity of EXTRACT and ESSENTIAL OIL of ARTEMISIA AUCHERI collected from Iran.Materials & Methods: In this sectional study, the compositions of ESSENTIAL OIL and ethanolic EXTRACT of ARTEMISIA AUCHERI were determined by GC/MS and HPLC assays. Antibacterial activity of the ESSENTIAL OIL and EXTRACT was evaluated using MICro broth dilution and disc diffusion methods.Results: The main component of the oil was, 1.8 Cineol (22.65%), and the main polyphenolic compounds of the EXTRACT was chloregenic acid (264.9 mg/l). Evaluating MIC and MBC values of the ESSENTIAL OIL, the maximum inhibition zone diameter (in the range of 6.4-8 mm, and 6.4-9 mm) was measured against Bacillus subtilis, Brenneria nigrifluens, Streptomyces scabies, Rhizobium radiobacter, Rhizobium vitis, Xanthomonas axonopodis pv. citri, and Xanthomonas arboricola pv. juglandis. Furthermore, evaluating MIC and MBC values of the EXTRACT the maximum inhibition zone diameter (in the range of 6.4-8 and 6.4-9 mm) was observe against Rhizobium Radiobacter, Brenneria nigrifluens, Rhizobium vitis, Streptomyces scabies, Bacillus subtilis, Xanthomonas axonopodis pv. citri, Xanthomonas arboricora pv. Juglandis, and Ralstonia solanacearum.Conclusion: In general, both ESSENTIAL OIL and ethanolic EXTRACT showed desirable antimicrobial activity against plant photogenic bacteria. Therefore, ARTEMISIA AUCHERI can be considered as a biopesticide.

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