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Title

EFFECT OF LEDERMIX AND A NEW FORMULATED MEDICAMENT ON ENTEROCOCCUS FAECAILS (IN VITRO)

Pages

 Start Page 235 | End Page 240

Abstract

 Background and Aim: Enterococcus faecails has been realized to have a key role in post endodontic infections. Treatment strategies designed to eliminate this microflora should include agents that can effectively disinfect the root canal. The purpose of this study was to evaluate the anti-microbial effect of different concentrations of Ledermix and a new medicament on Enterococcus faecails.Materials and Methods: In this experimental in vitro study, E.faecails was inoculated to BHI (Brain Heart Infusion) broth and incubated at 37o C for 24 hours. The cell suspension of E. faecalis was then spread on to a blood agar plate and was incubated overnight at 37oC for 24 hours. One colony of microorganism was inoculated to TSB media and incubated overnight at 37o. One hundred microliters of the suspension was spread thoroughly onto a Moller Hinton Agar. Thirty Prepared disks with in different concentrations were fixed on to plates and incubated overnight at 37o C. The latest loop culture that did not show any growth was considered as MIC. and any concentrations of MIC that didn't show growth on agar plates was considered as MBC. The results were analyzed using one way analysis of variance (ANOVA).Results: The average inhibition zone for bacterial growth was measured to be 2.56±0.20cm for ledermix in concentration of 1gr/lit, and 2±0/30cm in concentration of 0.5g/lit. The inhibition zone measurement was 1.53±0.40cm for new formulation medicament in concentration of 1 gr/lit. The difference was statistically significant (p<0.05). MIC effect of ledermix revealed in in concentration of 0.5 gr/lit and for new formulation medicament was in concentration of 1gr/lit.Conclusion: Both compounds display similar anti-microbial effects. However, Ledermix prepared at higher concentrations appears to have a greater inhibitory effect on growth of E.Faecalis.

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