Journal Paper

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Journal: کشاورزی پویا
Year:1387 | Volume:5 | Issue:2
Start Page:229 | End Page:243

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Title

DETERMINATION OF RELATIVE POPULATION OF BIFIDOBACTERIUM SPP. IN BROILER GUT USING DENSITOMETRY TECHNIQUE BASED MOLECULAR GENETICS

Pages

 Start Page 229 | End Page 243

Abstract

 Presently, modern molecular techniques are used for investigation on gastrointestinal bacteria. BIFIDOBACTERIUM SPP. Has a critical role in prevention of some pathogens colonization in gastrointestinal tract's POULTRY. These bacteria increase POULTRY resistance against diseases and improve their immune system and performance. This experiment was conducted in order to determinine relative population of BIFIDOBACTERIUM SPP. in duodenum, jejunum, ileum and cecum of broiler chickens at 2007 in Islamic Azad University, Rasht Branch. gut contents it were removed and extracted their DNA. It was obtained a a specific band for detecting of mentioned bacterium using a set specific primer and also a specific band for detecting of all bacteria. Relative population of BIFIDOBACTERIUM SPP. It was determined relative to total gut bacteria. analysis showed BIFIDOBACTERIUM SPP. consists 4.50% of total duodenum bacteria. Also it was shown that BIFIDOBACTERIUM SPP. consists 4.95% of total jejunum bacteria. Meanwhile BIFIDOBACTERIUM SPP. consists 5.17% of total ileum bacteria. Furthermore it was shown that BIFIDOBACTERIUM SPP. consists 9.36% of total cecum bacteria. Relative population of BIFIDOBACTERIUM SPP. in lower segments i.e. ileum and cecum were higher than upper segments i.e. duodenum and jejunum. Meanwhile, relative population of BIFIDOBACTERIUM SPP. in 4, 14 and 30d of ages in cecum were higher than other segments i.e. duodenum, jejunum and ileum. results showed that BIFIDOBACTERIUM SPP. populations were variable in various intestine segments and correlated with their functions and physicochemical environment.

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