Journal Paper

Paper Information

Journal: JOURNAL OF CROP ECOPHYSIOLOGY (AGRICULTURE SCIENCE) | Year:1388 | Volume:3 | Issue:11 | Start Page:39 | End Page:46

video

sound

Persian Version

View:

346

Download:

113

Cites:

Information Journal Paper

Title

MOLECULAR INVESTIGATION OF BLAD DISORDER IN BREEDER BULLS OF WEST AND NORTH-WEST ANIMAL BREEDING CENTER

Pages

 Start Page 39 | End Page 46

Abstract

 Autosomal recessive genetic diseases in cattle are breed-specific and one of them is bovine leukocyte adhesion deficiency syndrome (BLAD). It is a genetic disease that affects especially the HOLSTEIN breed. The molecular basis of BLAD is a single point mutation (adenine to guanine) at position 383 of the CD18 gene, which caused an aspartic acid to glycine substitution at amino acid 128 (D128G) in the adhesion molecule CD18. Recognition of these disorders presently was carried out by PCRRFLP with TaqI and HaeIII enzymes. In this research, DNA samples purified from bulls and then investigated the quality and quantity of extracted DNA.101bp of CD18 gene amplificated and electrophoresis carried out on 2% agaros gel. Digested products run on 10% polyacrylamide gel and then stained by etidium bromide. None of the bulls under study were found to be BLAD carrier and all recognized as TL code.

Cites

  • No record.
  • References

  • No record.
  • Related Journal Papers

    Related Seminar Papers

  • No record.
  • Related Plans

  • No record.





  • Advertising

    مرکز اطلاعات علمی SID
    مرکز اطلاعات علمی SID
    مرکز اطلاعات علمی SID
    مرکز اطلاعات علمی SID
    مرکز اطلاعات علمی SID
    مرکز اطلاعات علمی SID
    مرکز اطلاعات علمی SID
    مرکز اطلاعات علمی SID