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Title

FATTY ACID COMPOSITION OF SACCHAROPOLYSPORA ERYTHREAEA GROWN INDEFINED MEDIUM

Writers

RAFIEINIA R. | ATAR H.

Pages

 Start Page 9 | End Page 14

Abstract

 Background and objective: SACCHAROPOLYSPORA ERYTHRAEA is a member of the aerobic and gram positive actinomycetes and the industrial microorganism for erythromycin production .It has shown that biomass composition of any microorganism and production of secondary metabolites strongly depends on composition of culture medium.Materials and methods: Spore suspensions of SACCHAROPOLYSPORA ERYTHRAEA PTCC1685 were inoculated into flasks containing seeding medium and incubated at 30°C for 40-44 h with 180 rpm in aerobic condition. The appropriate amount of seeding cultures that showed suitable morphology, pH and growth were added into flasks containing fermentation medium. Flasks were inoculated at 30°C for for 11 days with 180 rpm. Biomass in stationary phase had separated by sentrifugation in 4000 rpm for 20 min. For lipid extraction, biomass is homogenized with chloroform/methanol (2/1) to a final volume 20 times the volume of the biomass .After dispersion, the whole mixture is agitated during 12h in an orbital shaker at room temperature. Then the mixture was sentrifuged at 4000rpm for 20 min. Upper phase removed by siphoning. The lower chloroform phase containing lipids evaporated under vacuum in rotary evaporator. Methyl esters of FATTY ACIDS of lipids prepared for GC-MASS analysis.Results: Eight FATTY ACIDS were detected which contained 15 to 18 carbon atoms. 14-methyl pentadecanoic acid (iC16:0) was in the highest percentage (36.25)% and pentadecanoic acid (aC17:0) were in the lowest percentage (1.64.)% Other FATTY ACIDS were C17:0(18.9)%, C18:2(14.99)%, C16:0(8.85)%, C18:1 (8.67)%, C18:0(5.47 )% and C15:0 (5.2.)% FATTY ACIDS with less than 15 carbon atoms had not detected in this study. Conclusions: Fatty acid composition and erythromycin production in S.erythreae strongly depends on culture composition.

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