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Title

EFFECTS OF ABSCISIC ACID, MANNITOL AND TEMPERATURE ON IN VITRO STORAGE OF POTATO GERMPLASM

Writers

 ARVIN M.J. | RAHIMI MARYAM

Pages

 Start Page 187 | End Page 196

Abstract

 In vitro storage of eight POTATO GENOTYPES (four cultivars and four wild species) using either abscisic acid (ABA) (5 mg 1-1) or MANNITOL (3%) as growth retardant added to Murashige and Skoog (MS) medium was examined at two storage temperatures (8oC and 25oC). After 10 months, the cultures were evaluated. Compared with control, medium containing ABA or MANNITOL significantly retarded growth. Genotypes were significantly different for most characteristics - measured with cultivars 'Cosima' and 'Draga' superiority to others. The survival rate was significantly affected by culture medium and was the highest with MANNITOL treatment. Significant two-factor interactions for some characters and also different responses of cultivars and wild species for regeneration following transfer to normal condition may complicate the establishment of facilities for IN VITRO STORAGE. However, MS medium supplemented with 3% MANNITOL showed to be superior for storing potato germplasm in vitro at room temperature.

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