Journal Paper

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Journal: گیاهان دارویی
Year:1395 | Volume:15 | Issue:58
Start Page:110 | End Page:119

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Title

THE EFFECT OF L-TYROSINE ON THEBAINE PRODUCTION IN CELL SUSPENSION CULTURE OF IRANIAN POPPY (PAPAVER BRACTEATUM)

Pages

 Start Page 110 | End Page 119

Abstract

 Background: Using of ELICITORs and different compounds in cell suspension culture is an effective strategy for enhancement and improvement of important SECONDARY METABOLITEs production in in vitro conditions.Objective: Evaluation of the effects of L-tyrosine and its combination with biological ELICITORs such as chitosan and salicylic acid on thebaine production in Iranian poppy cell suspension cultures at different days after elicitation.Methods: Different concentrations of L-tyrosine (1 and 2 mM) individually and in combination with chitosan (100 mg/L) and salicylic acid (50 mg/L) was applied on cell cultures, and then, pH and EC of medium, and also thebaine content of cells were measured on 2, 4 and 6 days after treatment.Results: The highest thebaine content of cells (42.30 mg/L) was obtained in 1 mM L-tyrosine on six days after treatment, which was significantly higher than the control. In addition, there was a relation between thebaine content of cells and pH and ECof culture medium. With increasing the thebaine content of cells, pH of medium was increased and EC of medium was decreased.Conclusion: Feeding of Iranian poppy cell suspension cultures with L-tyrosine (precursor of alkaloids biosynthesis) was improved thebaine production. So that, 1 mM L-tyrosine increased the thebaine content of cells up to 22.26-fold compared with control.

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