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Title

INFLUENCE OF MICROENCAPSULATION OF INDIGENOUS PROBIOTIC LACTOBACILLUS PLANTARUM ON BACTERIAL SURVIVAL IN SIMULATED GASTROINTESTINAL CONDITIONS

Pages

 Start Page 463 | End Page 472

Abstract

 During the last two decades, too many studies have been assigned to lactic acid bacteria (LAB) detection and identification of native strains of LAB. The aim of this study was to examine the efficacy of capsulation to carry native LAB, Lactobacillus plantarum (KC355240), and bacteria survival in SIMULATED GASTROINTESTINAL CONDITIONS. The native Lactobacillus plantarum was activated and microencapsulated using calcium alginate, and then the size and morphology of the particles was determined. Then the ability of probiotic bacteria survival through passing the SIMULATED GASTROINTESTINAL CONDITIONS (pH=2 pepsin and pH=7.4 bile salt solution) was investigated. Morphology of the beads using SEM showed globular capsules and this indicating positive effect of alginate in order to form a steady wall for these bacteria. In addition, improving survival ability of bacteria through passing gastrointestinal system simulation indicated appropriate substance of calcium alginate to microencapsulating. The results indicate that this method of MICROENCAPSULATION lead to generate the same, regular and spherical beads with diameter less than 100 micrometers and also microencapsulating with calcium alginate will improve survival of Lactobacillus plantarum bacteria through passing digestive system in about four logarithmic cycles.

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