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Information Journal Paper

Title

EXAMINATION OF DIGESTIBILITY OF PROCESSED BARLEY GRAIN WITH DIFFERENT METHODS, USING GAS PRODUCTION TECHNIQUE WITH TWO SOURCES OF INOCOLA

Author(s)

PARAND E. | TAGHIZADEH AKBAR

Pages

  1-13

Abstract

 In this study the GAS PRODUCTION technique was carried out using two sources of inocola (rumen and feces liquor from sheep) firstly to characterize the effect of four different PROCESSING methods on digestibility of untreated BARLEY grain (UBG) and treated BARLEY grain (SRBG = steamrolled BARLEY grain, MWBG = micro waved BARLEY grain, RSBG = roasted BARLEY grain and MLBG = milled BARLEY grain), and secondly to find out the accuracy of using feces a as inocola in GAS PRODUCTION technique to evaluate concentrate feedstuff. In in-vitro GAS PRODUCTION technique triplicate grounded and dried samples of each treatment (approximately 300mg) were weighed and placed in 50 ml capacity serum bottles; then bottles were incubated in 20 ml of  buffered rumen fluid/faecal liquor (buffer: rumen fluid, 2:1, v/v) for 48 h. The data at the different times was analyzed using completely randomized design. The results showed that in rumen liquor at 48 h microwave PROCESSING group had the most and steam rolled BARLEY grain had the least total GAS PRODUCTION (275.9 and 239.14 ml/gDM, respectively) (P<0.05), but the GAS PRODUCTION at 48 h using feces as inocola, did not significant difference. Percentage of organic matter digestible in MWB and UBG (65.14 and 65, respectively) was maximum compared to RSGB and SRGB (58.21 and 57.83, respectively) was achieved. Also we found a high relationship (r2=0.9) between GAS PRODUCTION (ml/mg DM) with two sources of inocula which suggests that feces can be used successfully in GAS PRODUCTION technique to evaluate concentrate feedstuffs. The results suggest that because of considerations of disorders like acidosis or bloat, use of PROCESSING like steam flaking and roasting in order to bypass some of starch to the small intestine can be helpful. The data showed that the faecal liquor can be used as a replacement assay for rumen liquor in GAS PRODUCTION technique resulting in low costs.

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