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Title

THE EFFECT OF SELENIUM AND VITAMIN E SUPPLEMENT ON THE SERUM CONCENTRATIONS OF ANTIOXIDANT AND F2 ISOPROSTANES IN ARABIAN HORSES DURING THE TRAINING SEASON

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 Start Page 75 | End Page 87

Abstract

 This study was conducted in order to evaluate of premix included SELENIUM and VITAMIN E effect on serum antioxidant (SELENIUM, GLUTATHIONE PEROXIDASE and VITAMIN E) and F2 ISOPROSTANES (marker of oxidants) on 40 Arabian male horses. Animals were located in control and treatment groups. Control group received only routine equine diet. Treatment group received routine diet plus premix (included 0.5 mg sodium selenite+250 mg a -tocopherol) for sixty days. Blood samples (5ml) were taken by venoject tube from jugular vein on day 0, 30 and 60 of trail period. Blood sera SELENIUM, GLUTATHIONE PEROXIDASE, VITAMIN E and F2 ISOPROSTANES concentrations were measured using atomic absorption, biochemical, fluorimetric chromatography and ELISA respectively. The mean of measured parameters did not show any considerable changes at study period (0 – 60 day) in control group, But sera F2 ISOPROSTANES decreased and other metabolites increased significantly in treatment group. the mean of sera SELENIUM, GLUTATHIONE PEROXIDASE, VITAMIN E and F2 ISOPROSTANES concentrations had significant difference between control and treatment groups on 30 and 60 day. On day 30 the correlation of SELENIUM and GLUTATHIONE PEROXIDASE was significant in both groups (P<0.001 and r=0.961; P<0.001 and r=0.844 respectively).the correlation of SELENIUM and GLUTATHIONE PEROXIDASE was significant in control group (P<0.001 and r=0.940), but the correlation of SELENIUM and GLUTATHIONE PEROXIDASE (P<0.001 and r=0.727) and the correlation of SELENIUM and F2 ISOPROSTANES (P<0.05 and r=- 0/530) was significant in treatment group at day60. The results of present study showed that use of VITAMIN E and SELENIUM premix increased blood sera antioxidants and decrease F2 ISOPROSTANES, according to results, use of premix is important in horse especially during exercise.  

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