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Title

MOLECULAR PURITY ASSESSMENT OF RICE CMS LINES

Writers

KIANI G. | NEMATZADEH G.

Pages

 Start Page 149 | End Page 153

Abstract

 The cytoplasmic male sterile (CMS) lines that are used in production of hybrid seeds in three-line system often get contaminated with their maintainer lines. Using such impure CMS lines in RICE hybrid seed production lead to un-uniformity and yield loss in hybrid RICE. Laborious and time consuming phenotypic evaluations are used for elimination of off-type plants in CMS multiplication farms. The other option for off-type elimination in CMS lines is application of molecular markers. In this study, PCR-based MITOCHONDRIAL MARKERS was used for distinguishing five male sterile lines from five maintainer lines. Results showed that marker cms had ability to differentiate male sterile lines Neda A and Nemat A from their maintainers. Also, marker drrcms differentiated male sterile lines Dasht A, Champa A and Amol 3 A from their respective maintainers. Results of the study demonstrate use of these two markers for off-type detection in CMS seed multiplication process. This is helpful in promotion of hybrid seed production technology in Iran.

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