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Information Journal Paper

Title

THE EFFECT OF PROTEIN AND DNA FRACTION OF TOXOPLASMA GONDII ON NITRIC OXIDE PRODUCTION AND GROWTH AND SURVIVAL OF PERITONEAL MACROPHAGES

Pages

  7-13

Abstract

 IntroductionTOXOPLASMA GONDII is an obligate intracellular parasite that invades a wide variety of host cells including MACROPHAGEs and survives within it. The parts of Toxoplasma that participate in the mechanism of its evasion from immune system and MACROPHAGE defenses are not completely defined. In this study, we evaluated the effect of protein and DNA Toxoplasma fractions on proliferation and NITRIC OXIDE production by peritoneal MACROPHAGEs.Material and MethodsThe viability of MACROPHAGEs was evaluated using3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) reduction assay and the production of nitrite using Griess method.ResultsMTT reduction and hence the growth and viability of MACROPHAGEs in a dose of 200 ng/ml was significantly lower than those of the negative control (P=0.022); in other lower doses of protein it was not statistically significant (P>0.05). Different doses of Toxoplasma protein fraction did not affect NO production (P>0.05). MTT ASSAY and NO production in different doses of DNA fraction was not different (P>0.05).ConclusionAccording to the results of the present study, protein fraction of Toxoplasma has a suppressive effect on MACROPHAGE viability, but this effect is dose dependent. Protein fraction of Toxoplasma does not affect the amount of NO production by MACROPHAGE. The isolated DNA fraction of Toxoplasma did not influence the viability and NO production of MACROPHAGEs. So, the ability of evasion of TOXOPLASMA GONDII from MACROPHAGE defense is due to a component of its protein.

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