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Information Journal Paper

Title

INVESTIGATION OF GENETIC DIVERSITY IN PERSIAN CUMIN [BUNIUM PERSICUM (BOISS.) B. FEDTSCH.] GERMPLASM FROM KERMAN PROVINCE USING RAPD MOLECULAR MARKERS

Pages

 Start Page 414 | End Page 427

Keywords

PERSIAN CUMIN [BUNIUM PERSICUM (BOISS.) B. FEDTSCH.]Q3

Abstract

 Persian Cumin, a medicinal and aromatic plant, belongs to Apiaceae family. The objective of this study was to assess the genetic variation in GERMPLASM of PERSIAN CUMIN [BUNIUM PERSICUM (BOISS.) B. FEDTSCH.]ollected from KERMAN province using RAPD molecular markers. The PERSIAN CUMIN [BUNIUM PERSICUM (BOISS.) B. FEDTSCH.]ruits were collected from 43 various regions in KERMAN province. DNA was extracted from fruits using modified CTAB method. From the 27 used primers in PCR, 19 primers with better bands were selected for analysis. The 446 polymorphic bands obtained by these primers were scored. The binary matrix was converted to distance matrix by applying Dice similarity coefficient in NTSYS-pc (Ver 2.02) software. Then, the distance matrix was analyzed using UPGMA and the phylogenic dendrogram was plotted. Based on the results, investigated populations were clustered in 7 groups. The obtained clusters based on RAPD markers to some extent matched with the geographical origin of the studied populations of Persian Cumin. Furthermore, the obtained results of principal component analysis method were similar to the results of cluster analysis. The results indicated that RAPD technique is an efficient tool for assessing genetic diversity in these populations.

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