The Alburnus chalcoides is of the species of teleost fish of the Caspian Sea. Sampling was done randomly on days 1 to 5 and days 7, 8, 10, 15, 20, 25, 30, 40, 50, 60, 75, 90 after hatching, pre-puberty and puberty. The digestive enzymes studied in this fish include pancreatic enzymes (trypsin, chtotripsin, lipase, amylase) and intestinal enzymes (alkaline phosphatase and N-aminopeptidase). During the growth of fish, trypsin, kumotrypsin and N-aminopeptidase enzymes had an increasing trend which is caused by a high-protein diet. The phosphatase enzyme also has a rising trend up to one month of age and is then fixed under the effect of the pH of the environment. Amylase and lipase enzymes have an incremental reduction peak which shows that the fish diet has low sugar and fat compounds. Significant differences were observed for all enzymes (P≤ 0. 05) which has been the start of a significant difference for the trypsin and chomitripsin from the third day, lipase enzyme from the second day, amylase enzyme from the seventh day and the alkaline phosphatase and N-aminopeptidase from day five. All the digestive tract enzymes studied in this fish on the day of onset of active feeding has had incremental peak.