Purpose: To evaluate visual outcomes and intraocular pressure (IOP) after Single-site phacotrabeculectomy with mitomycin C in patients with senile cataract and primary open angle glaucoma (POAG), pseudoexfoliative glaucoma (PXG), or chronic angle closure glaucoma (CACG).Methods: In this retrospective study, patients older than 50 years who had significant cataracts (BCVA≤20.60) and uncontrolled POAG, PXG, or PACG (IOP>21 mmHg with at least two anti-glaucoma medications) were enrolled.Exclusion criteria included the presence of corneal pathologies, uveitis, diabetic retinopathy, neovascular or phacomorphic glaucoma, posterior segment pathologies, or a history of ocular trauma or intraocular surgery.Preoperative examination included uncorrected and best-corrected visual acuity (UCVA and BCVA, respectively), slit lamp biomicroscopy, IOP measurements, gonioscopy, and dilated funduscopy. The participants underwent single site phacotrabeculectomy with mitomycin C. Postoperative follow-up examinations were scheduled on days 1, 3, 7, and 14, and months 1, 3 and every 3 months thereafter. In each follow-up examination, the patients were re-evaluated for BCVA, IOP, the number of anti-glaucoma medications if needed, appearance of the bleb, and postoperative complications.Results: Forty (24 right) eyes of 32 (12 female) patients with POAG (15 eyes), PXG (18 eyes), or CACG (7 eyes) were enrolled. Mean patient age was 73±9 (range, 46 to 88) years and the study groups were comparable in this regard (P=0.88). Mean postoperative follow-up period was 13.1±9.5 months, raging from 1 to 40 months. Of the participants, 6 had diabetes mellitus and 6 had systemic hypertension. Mean preoperative BCVA was 0.64±0.32 logMAR (20/80) which improved to 0.38±0.19 logMAR (20/50) postoperatively (P<0.001). Preoperatively, mean IOP was 18.6±6.0 (range, 7 to 33) mmHg which was decreased to 12.3±3.0 (range, 6 to 17) mmHg postoperatively (P< 0.001).The PXG group demonstrated the highest reduction (P=0.001) and the POAG group experienced the lowest reduction (P=0.021) in IOP. At the last follow-up examination, the number of anti-glaucoma medications was reduced to 0.9±1.1, on average (P<0.001). The reduction in the number of medication was highest in the POAG (2.1±1.1, P=0.001) and lowest in the CAGC group (1.3±0.5, P=0.08). At final follow-up IOP was controlled with no medications in 21 eyes (52.5%), with timolol in 8 eyes (20%), and with 2 or more medications in 11 eyes (27.5%). With respect to intra- and postoperative complications and the appearance of the bleb, the study groups were comparable.Conclusion: One-site phacotrabeculectomy with mitomycin C is a safe and effective approach to decrease IOP and improve BCVA in patients with coexisting cataract and glaucoma.