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مرکز اطلاعات علمی SID1
اسکوپوس
دانشگاه غیر انتفاعی مهر اروند
ریسرچگیت
strs
Issue Info: 
  • Year: 

    2016
  • Volume: 

    27
  • Issue: 

    4
  • Pages: 

    305-312
Measures: 
  • Citations: 

    0
  • Views: 

    56432
  • Downloads: 

    36774
Abstract: 

One of the most commonly observed surface structures on the prokaryotic cell envelopes is monomolecular crystalline array of proteinaceous subunits termed Surface Layers or S-layers. Due their self assembly ability and the highly ordered, regular structure down to the nanometer scale, S-layers were demonstrated to possess a great potential for nanobiotechnological applications. Some probiotic bacteria, such as Lactobacillus spp. have been found to possess S-layers. In this perspective, the objectives of this study were isolation and identification of Lactobacillus species from traditional dairy products carrying surface (S) layer protein and investigation of important prerequisite of probiotic interest, such as the capability to survive at low pH and in presence of bile salts. The protein profile of intact Lactobacillus isolates was analyzed in SDS-PAGE. The protein bands of 45– 60 kDa were present in two of the 25 isolates, suggesting the presence of S-layer proteins in these strains. The above mentioned proteins were recovered in the guanidine hydrochloride extracts. After dialysis, the extracted proteins showed a significant band of apparent molecular mass of 50– 60 kDa in SDS-PAGE. The strains possessing S-layers protein was identified both biochemically and by 16S rRNA sequencing. The 16S rRNA gene sequence analysis revealed that two isolates exhibited maximum similarity with the 16S rRNA sequence of Lactobacillus brevis spp. These two isolates were resistant to acid and bile in which their viability in acidic condition were between 51. 6% and 77. 8% and bile resistance of two isolates was approximately 99% after 24 h.

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Author(s): 

RAHNAMA H. | SHEYKHHASAN M.

Issue Info: 
  • Year: 

    2016
  • Volume: 

    27
  • Issue: 

    4
  • Pages: 

    313-319
Measures: 
  • Citations: 

    0
  • Views: 

    37735
  • Downloads: 

    15335
Abstract: 

Rapeseed (Brassica napus L. ) is the third most important oil crop in global productions. One of the major limiting factors for oilseed rape production is lepidopteran pests of the Brassicaceae family. Transgenic plants expressing Bacillus thuringiensis (Bt) genes are powerful tools in the integrated pest management of crop plants. In the present study, we used a synthetic Bt insecticidal crystal protein gene (cry1Ab) under the control of the phosphoenolpyruvate carboxylase (PEPC) promoter for genetic transformation of B. napus L. (var. SLM046) using Agrobacterium tumefaciens-mediated transformation. PEPC-cry1Ab-nos cassette cloned in a binary vector pCAMBIA3300 containing bar gene as a selection marker. The new vector (pCAMBIAPEPCry) introduced to AGL01 strain of A. tumefaciens, which used in transformation of hypocotyl explants of B. napus. Putative transgenic rapeseed plants were regenerated in selection media containing phosphinothricin (PPT) as selection agent. Polymerase Chain Reaction (PCR) confirmed the integration of cry1Ab and bar genes at putative transgenic plant genome. Furthermore, transcription (mRNA production) and protein expression of cry1Ab gene was confirmed using RT-PCR and immune-strip methods, respectively. Transgenic B. napus plants expressed Cry1Ab protein in the shoots and not in the roots. We concluded that C4 maize PEPC promoter can induce the expression of Cry1Ab recombinant protein only in light treated (green) tissues in rapeseed plants. It recomended as a light inducible promoter for targeted expression of transgene in the rapeseed plants.

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Author(s): 

BALUJA SH. | Ramavat p. | Nandha k.

Issue Info: 
  • Year: 

    2016
  • Volume: 

    27
  • Issue: 

    4
  • Pages: 

    321-328
Measures: 
  • Citations: 

    0
  • Views: 

    74592
  • Downloads: 

    37458
Abstract: 

The acidity constants of some synthesized protonated pyrazolo quinazoline compounds were determined potentiometrically at ionic strength of 0. 1 M in DMF: water (60: 40 v/v) system at different temperatures (298. 15 K to 318. 15 K). The pKa values have been found to increase with increasing electron-donating nature of substitutions. Some thermodynamics parameters such as enthalpy (Δ H° ), Gibb’ s free energy (Δ G° ) and entropy (Δ S° ) of reactions have also been evaluated at different temperatures for these systems. The thermodynamic parameters indicate that dissociation process is not spontaneous and exothermic.

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Author(s): 

Ehteshami Moinabadi M.

Issue Info: 
  • Year: 

    2016
  • Volume: 

    27
  • Issue: 

    4
  • Pages: 

    329-342
Measures: 
  • Citations: 

    0
  • Views: 

    69092
  • Downloads: 

    59383
Abstract: 

Transverse basement (TB) faults are important structures in the mountain belts or sedimentary basins influencing various aspects of them. The origin of these faults is diverse, but their effect on the shape and configuration of continental margins is characteristics. The Western Alborz range that borders the South Caspian basin to southwest is a complex range with principal faults and known earthquakes such as 1990 Rudbar (Mw 7. 3). However, TB faults are less known in this range. This paper attempts to compile available data from aeromagnetic survey, local geology maps, tectonic maps, remote sensing and earthquake data to introduce several important TB faults or lineament in the Western Alborz. Qezel Owzan-West Talesh, Lahijan-Sepidrud, Takestan-Polrud and Valian-Hezar are large TB faults or lineaments across the Western Alborz. Some of introduced TB faults and lineaments are possibly continuing within the South Caspian basin. The TB faults introduced or assessed in this paper are more or less correlating with irregularities of the northern margin of the Alborz Range. This correlation is clearer for the Lahijan-Sepidrud TB fault, F-1lineament and to a lesser degree for Amlash, Polrud-Takestan and Valian-Hezar lineaments. Detail geometry, origin, effect on sedimentation along the continental margin and their relationship with huge regional magmatism in the Cretaceous and Eocene in the Western Alborz suggested subjects for further investigations on these faults and lineament.

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Author(s): 

Jamshidibadr m.

Issue Info: 
  • Year: 

    2016
  • Volume: 

    27
  • Issue: 

    4
  • Pages: 

    343-352
Measures: 
  • Citations: 

    0
  • Views: 

    41234
  • Downloads: 

    29075
Abstract: 

Turkeh Dareh pluton is outcrop in NW of Iran and it is one of the intrusions in Sanandaj-Sirjan zone. Shaped crystals of this pluton distinct oscillatory zoning narrow and close together zoning in zircons indicate parental magma richness of zirconium. Factors affecting the shape of the zircon crystals are the composition, possibly the temperature of the crystallization, water content in magma and origin of the magma, therefore, were studied morphology of zircon crystals of Turkeh Dareh pluton. Morphologically, most of Turkeh Dareh zircons are located in range of S13 and S14 and in lesser extent in the range of S18, S19 and S20. The minimum zircons crystallization temperature was calculated 750 to 840 Cº based on the morphology of zircon, zircon saturation and the whole rock geochemistry. The origin of intrusion magma of Turkeh Dareh is I-type and calc-alkaline that these results are consistent with results of field observations, mineralogy and geochemistry of this pluton.

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Issue Info: 
  • Year: 

    2016
  • Volume: 

    27
  • Issue: 

    4
  • Pages: 

    353-355
Measures: 
  • Citations: 

    0
  • Views: 

    64434
  • Downloads: 

    33012
Abstract: 

One of the important problems in group theory is characterization of a group by a given property, that is, to prove there exist only one group with a given property. Let G be a finite group. We denote by k(G) the largest order of elements of G. In this paper, we prove that some Suzuki groups are characterizable by order and the largest order of elements. In fact, we prove that if G is a group with |G| = |Sz(q)| and k(G) = k(Sz(q)) where q − 1 or q ± 2q + 1 is a prime number, then G ≅ Sz(q).

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Author(s): 

Jafaraghaie r. | NEMATOLLAHI N.

Issue Info: 
  • Year: 

    2016
  • Volume: 

    27
  • Issue: 

    4
  • Pages: 

    357-366
Measures: 
  • Citations: 

    0
  • Views: 

    49217
  • Downloads: 

    26470
Abstract: 

One of the most important prediction problems in finite population is the prediction of a linear function of characteristic values of a finite population. In this paper the admissibility of linear predictors of an arbitrary linear function of characteristic values in a finite population under reflected normal loss function is considered. Under the super-population model, we obtain the conditions for which the linear predictors are admissible. Also, the risk of some admissible and inadmissible predictors are compared by a simulation study.

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