VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ)
Year:2009 | Volume:3 | Issue:2 (10)

In this research, plasma homocysteine levels as an independent risk factor of cardiovascular disease was evaluated in streptozotocin induced diabetic New Zealand white rabbits. Twelve male New Zealand white rabbits were selected and allocated into two separate groups of test and control. Following confirmation of the rabbit’s health status (normal plasma glucose, urea and creatinine values), those in the test group received a single dose of streptozotocin at 65 mg/kg through the marginal ear vein while the control group were given normal saline solution. Blood samples were collected from the marginal ear vein after 24, 48 and 72 hours and then once every week for 12 weeks and plasma homocysteine, insulin and glucose levels were measured and statistically evaluated. The results indicated significant increase (p<0.01) of plasma homocysteine and glucose levels and significant decrease (p<0.01) of plasma insulin levels of the treatment group in comparison with the control group throughout the 12 week study period. In the present study, despite the decrease in plasma insulin level, increase of plasma homocysteine was observed as an independent risk factor of cardiovascular disease in New Zealand white rabbits.

Colorectal adenocarcinoma is one of the most prevalent and treatable malignancies of the gastrointestinal tract. Recent studies on colorectal neoplasia indicate b-catenin gene mutation and its intranuclear accumulation inside hyperplastic cells. Therefore, b-catenin may be an important prognosis and diagnostic index of this disease. The aim of this study is to evaluate the nuclear beta-catenin expression in hyperplastic cells of colon following treatment with 1,2-dimethylhydrazine. In this study, 56 wistar male rats with the age of 12 weeks and body weight of 200-300g were selected and randomly allocated into two equal treatment and control groups. In the treatment group, two subcutaneous injections of 1,2-dimethylhydrazine every week at a dose rate of 40 mg/kg were used for 4 weeks to induce colon carcinoma. The control group was given normal saline solution similarly. After 8 weeks, tissues samples of distal colon were collected from both groups for preparation of tissue sections and immunohistochemistry. Immunohistopathological study of samples revealed that nuclear b-catenin expression in the treatment group was significantly higher than the control group (p<0.01). The study indicates that the expression of nuclear beta-catenin can increase in hyperplasia and neoplasia of colon.

Selective serotonin reuptake inhibitors (SSRIs) are medications prescribed for the treatment of mood disorders including anxiety and depression. These drugs can prevent the development of inflammation and modulate interleukin and interferon production. It can be assumed from these features that the use of SSRIs could have a significant effect on wound healing. The aim of this experimental investigation was to evaluate the effect of sertraline on dermal wound healing in an animal model. In 60 Wistar rats, two circular wounds were made using a biopsy punch on the back of each animal. Rats were assigned to five groups of twelve animals each: Control group; Placebo group and experimental groups of the low, moderate and high dosage of sertraline ointment. Four rats of each group were sacrificed at 3, 7 and 15 days after operation. The shapes of the wounds were copied onto a transparent sheet, the figures were scanned, and the wound areas were calculated using scion image software and statistically analyzed. For histopathological studies, regenerated tissues were cut in the form of square pieces along with normal skin and stained with hematoxylin and eosin. The results show that sertraline treatment significantly reduced mean wound length. Morphmetrically, there were statistically significant differences between control, Placebo and experimental groups and there were significant histopathological differences between control, Placebo and experimental groups at the end of the study. The results of this study demonstrated that topical use of sertraline significantly improves healing of cutaneous wounds in the rat.

Bovine Leukosis Virus (BLV) belongs to the genus Deltaretrovirus ,subfamily Orthoretrovirinae of the family Retroviridae comprising 3 main genes of gag, pol and env and a number of replication regulatory genes such as Tax, Rex, R III, C IV. For determination of genetic relationship of Tax gene of BLV in Iran with those in other countries fragments 927bp corresponding to Tax from four infected samples were amplified in PCR system and sequenced for determining nucleotide sequence and compared with identified nucleotide sequence of this gene in other countries.A comparison made on Tax gene in Iran with other countries demonstrated 3.4 to 7.7% variability in Tax gene, of which the greatest sequence similarity exists between sequences of Tax in Iran with USA (AY700378.1)with 96.6% similarity and the least relationship exists between sequences of this virus in Iran with Australia (AY700379.1) and Japan (AY700381.1) with 92.3% similarity.

With the significant increase in bottled natural mineral water production and consumption over the last decade in our country, there has been a growing concern over the microbiological quality of such products. The purpose of the present study was to analyze various bottled natural mineral waters sold in Tabriz. In this study, 150 samples of such products were examined for aerobic colony count, coliforms, fecal coliforms, Escherichia coli, fecal streptococci, Clostridium perfringenes and Pseudomonas aeruginosa. Aerobic colony count pseudomonas and clostridium were determined using membrane filtration method and most probable number (MPN) method was used for determining coliforms and fecal streptococci. The results indicated that in 25.3% of the samples there was no microbial growth in plate count agar media and 2% of the samples were contaminated with high levels of aerobic colony count (more than 20 bacteria/ ml). Furthermore, of the 72.6% of samples with acceptable aerobic colony count, 2.75%, 1.83%, 2.75% and 0.91% were contaminated with coliforms, fecal streptococci, pseudomonas and clostridium respectively.

Mycoplasma gallisepticum (MG) as one the major pathogens of birds, causes significant economic losses in poultry industry. The main purpose of the present study was to detect Mycoplasma gallisepticum in clinical samples using the 16S rRNA PCR method. For serological screaming test, 18 commercial laying farms and 8 broiler breeder farms were selected and rapid serum agglutination test (RSAT) was performed. For polymerase chain reaction sampling, 10 of the 17 farms that were positive in RSAT were selected and 109 sterile swab samples were collected from the palatine cleft, trachea, air sacs and lungs in each farm. Three swabs from three birds were placed in test tube containing 1 ml of phosphate buffered saline and transferred to laboratory form PCR testing. In this study, specific primers for 16S rRNA gene were used. The aforementioned primers are totally specific for MG and can be differentiated from other Mycoplasma and bacteria present in the trachea of poultry of the 26 farms examined, 17 farms were positive in RSAT serologic test. The 530 bp PCR product produced by specific primers of all field strains appeared on electrophoresis gel in 46 samples from 10 farms accounting to 42.2%.The 16S rRNA PCR with very high sensitivity can be employed in definitive diagnosis of Mycoplasma gallisepticum infection in vitro.

Considering that camel breeding is widespread in extensive parts of Iran and camels are important in transmission of some zoonotic disease to other ruminant and even humans, the necessity of conducting this study was assumed. In this research, apart from the rate of infection to blood parasites in camels of Zabol, the relationship between infection and some other factors including age, sex and geographical location of camel breeding were also studied. For this purpose, 113 blood samples of camels were taken from different regions of Zabol and transferred to the parasitology laboratory. The results indicated that 30.09% of studied camels were infected by blood parasites with the greatest infection rate of 19.47% belonging Trypanosoma evansi and then infection rates of 6.20% by Theileria sp., 3.54% by bacteria sp. and 88% by blood microfilaria were also observed. Statistical analysis did not show significant relationship between infection to blood parasites and age and sex of the studied camels. With regard to presence of blood parasites in the camels of the region and the importance of arthropods in their transmission, apart from treatment of infected animals, arthropod control measures should also be conducted in other to control these infections.   

Theileriosis is an important protozoal disease of domestic ruminants especially cattle, sheep and goats which is transmitted by various species of Theileria infected ticks. Anemia, electrolytic disorders and vasculitis are amongst the cardiovascular complications of theileriosis. In this study, 90 cows with theileriosis were evaluated by clinical examination, parasitologic tests and electrocardiography. In Theileria infected cattle, after 30 minutes of rest and any treatment, a 30 second ECG was recorded in lead I to determine the types of arrhythmias present. Based on electrocardiographic findings frequently observed arrhythmias included 62 cases of sinus tachycardia, 15 cases of sinus arrhythmia, 6 cases of first degree atrio-ventricular block, 1 case of ventricular extra systole and 1 case of atrial fibrillation. According to the type of arrhythmias, it can be concluded that arrhythmias in cows with theileriosis are functional and nonpathologic.