In many cases in concentrate factory of Sarcheshmeh copper co., the grade of copper in molibdenite concentrate exceeds its permissible limits. This causes economic reduction in value of product. So, with sodium cyanide addition, the copper content in concentrate is controlled. Sodium cyanide is very toxic and environmentally hazardous. In this investigation, studying the feasibility use of micro–organism ability is considered. At first after sampling of molibdenite concentrate of Sarcheshmeh Co., and chemical and mineralogical of obtained samples were under the bio-leaching process by mesophile bacteria mix culture of, (acidi thio bacilous thiooxidance acidi thibacilous ferroxidanse and leptosperilom feroxidance) and Thermophile (acidianous berili ,sulfo-lobous acido caldarious) bacteria.In this investigation, bio- leaching reactor and microbial study in shaking methods are considered. The results of microbial methods in shaking flasks, copper removal by mesophile bacteria is 55% and by thermophile bacteria is 75%. The results in bioleaching reactor showed, the rate of copper removal from molibdenite concentrate by mesophile bacteria is 50% (temp. 35°C, pH =2, solid % =10 and bacterial inoculation =12%) and by thermophile bacteria is 60% (temp.65°C, pH=2, solid%=10 and bacterial inoculation=12%). Therefore, the use of bacteria is important for copper removal from molybdenite concentrate. This approach also prevents contamination of the environment.