Sequoia is a very big and ever green tree with 45-90 meter height. It is native to the side of Atlantic Ocean, Which is also cultivated in Iran. The plant materials were collected from one of the Sequoia trees from Rezvanshahr Garden in August. The height to this tree was 40 meter and it was 30 years old.For preparation of tissue culture samples, the end part of shoots in 1-2 centimeter length has been cultivated.The leaves from main tree and also tissue culture sample were hydrodistilled for their essential oils. The oils were analyzed by combination of capillary GC and GC/MS.72 Compounds were identified in the leaf oil of main tree and 37 compounds were characterized in the leaf oil of tissue culture sample. The main components of the oil from main tree were b- phellandrene and limonene (13.30%), a- pinene (6.83%), terpinene -4-o1 (6.47%), g-teroubebe *5.44%) and germacrene B (4.17%), while the major components of the oil from tissue culture sample were a- pinene (26.30%), a- terpinenyl acetate (14.40%), sabinene (13.60%), g- terpinene (7.10%), germacrene D (6.90%), b-phellandrene and limonene (6.60 %) and p-cymene (5.00%). Althoug these components were found in both oils, but the higher percentage of some useful compounds in the oil of tissue culture sample, gave it more effective character.