Objective: To investigate the effect of chick embryo amniotic fluid (CEAF) as natural medium on the development of two-cell mouse embryos.
Design: Prospective experimental study.
Material and Method: 6-day and 10-day CEAF were collected separately and storaged at 4°C for a maximum of one week. Female mice superovulated, mated and two-cell embryos were flushed from the oviducts. Embryos were then randomly assigned to five treatment groups consisted of: nAF100, iAF100, iAF50, iAF20 as experimental groups and SA as control group. The embryos were cultured for 120h at 37°C in 5% CO2 and were assessed according to the stage of development.
Setting: Research laboratory associated with clinical IVF program.
Results: The percentage of morula, blastocyst and hatched blastocyst in CEAF were significantly higher than control group (P<0.05). The percentage of degenerated embryos in CEAF were significantly lower than control group (P<0.05). The speed of embryo development in CEAF was also higher than control group.
Conclusion: CEAF was able to support two-cell mouse embryos through developmental stages towards hatched blastocyst stage.