In the present study the effects of simoltaneous co-culture and sequential media on the development of vitrified one-cell mouse embryo were studied. Preimplantation embryos from several mammalian sepcies could be removed from the female tract and successfully vitrified and cultured in vitro. The zygot is very sensitive and need to have safe cryopreservation and a suitable culturing method after thawing. Experiments were conducted to find a suitable culture system for post-thaw of one-cell mouse embryos one-cell mouse embryos obtained from hyperstimulated mice oviducts and the embryos were divided into vitrified and unvitrified groups, as follows: 1) One-cell mouse embryos were vitrified with EFS 40% solution (ethylene glycol-ficoll-sucrose) and diluted with 0.5 M sucrose solution. Thawed embryos were transferred into MEMa and R1-R2 media with or without co-culture. [MEMa, MEMa+Vero, R1-R2, (R1-R2)+Vero, R1-(R2+Vero)]. 2) Fresh embryos were transferred to the same media except the last one [R1-(R2+Vero)]. Embryo developmental rate was scored during 120 h post-thaw. In vitrified groups, the highest developmental rate was seen in (R1-R2)+Vero and the lowest one was seen in MEMa medium. Fresh co-cultured embryos in MEMa medium, were able to vitrified developmental block, while embryos were blocked in MEMa. There was no significant difference in the developmental rate of non-vitrified embryos co-cultured with Vero cells comparing to those cultured on R1-R2 medium.