Introduction: Failure of implantation is one of the important factors of infertility. There is little information about human embryo implantation due to ethical and practical reasons. In addition, there is only minimal data available from primates and the use of some species may not give much information about the human because the process of implantation differs widely between species. Lack of attachment and invasion of embryo is the major factor of implantation failure. The aim of present study was establishment and study of attachment and invasion of embryo in vitro.
Methods and Materials: Surplus 4-8 cell human embryo resulted from ICSI were put on Matrigel until reached to blastocyst stage. Embryo secretion was collected in different stage of embryo and bHCG was measured by using ELISA. The relationship between embryo developments with the amount of bHCG secretion was investigated. Attachment of embryo was studied after hatching of embryo. Samples then were fixed in different time and invasion of embryo were examined.
Results: The results showed that bHCG secretion was detected from 8 cell embryo and this secretion was increased by embryo development. The amount of bHCG was significantly increased after hatching of embryo. The results showed that the 83% of hatched embryo could attach and 65% of them invade Matrigel.
Conclusion: The establishment of attachment and invasion of embryo in vitro could help us to understand inhibitory and inducing factors of attachment and invasion of human embryo and therefore it could help to prevent and increase implantation rate.