After several decades, the value of agrochemicals not only reduced but also as their detrimental effects for human and environment they became serious problem. Microorganisms are potential agents for plants diseases control. In this research the antagonistic effects of Pseudomonas isolates were studied on fungi Rhizoctonia. solani the cause of damping-off in tomato and other plants. Fungi isolated from plants and according to identification test isolates proved as R. solani. Among strains isolated from health tomato rhizosphere by use of biochemical test 26 strains identified as Pseudomonas spp. Proving the pathogenesis of fungi were done by introduce it to tomato seeds and isolate the fungi from contaminated plant. The antagonistic effects of bacterial isolates on the pathogenic fungi were studied on PDA agar. Two strains S5 and 22 could inhibit the fungi growth and around their colonies the non growing zone of fungi were seen. They also could inhibit the growth of fungi and cause the disease in tube and greenhouse studies. The growth promoting effects of these strains were studied in greenhouse. S5 and 22 strains increased the stem length of tomato 1.31 and 1.34 times more than control without bacteria respectively. Inoculation of S5 and 22 strains to seeds could increase dry weight of plant 1.66 and 2.33 times more than control respectively. As potential effects of these two strains for the inhibition of fungi and their enhancing effects for plant growth they are good candidates for use as BioControl agents and PGPB.